Magnetic Microspheres

We couldn't think of a good pun for "magnetic".

ProMag™

ProMag™ are highly uniform polymer-based magnetite spheres available in diameters of 1µm and 3µm. A unique surface means low nonspecific binding in protein-based systems, and superior handling without the use of surfactant. These high-binding beads are suitable for use across a range of research and diagnostic applications, whether you're working at laboratory scale or have the more stringent requirements of high throughput applications. You'll get noticeably decreased separation times, without breaking the bank!

ProMag™ are available with carboxyl, streptavidin, or Bind-IT™ surface functionalities. Bind-IT™ is a revolutionary surface chemistry that allows fast (≤60 minutes) and stable binding of antibody. The Bind-IT™ chemistry is designed to immobilize protein in a manner that preserves tertiary structure for optimal activity, which results in significant improvements in sensitivity, the dynamic range of immunoassays, and exceptional reproducibility between coating batches.

COMPEL™

COMPEL™ are highly uniform superparamagnetic microspheres ideal for applications that demand uniform particle response, such as miniaturized bioassays and separations. COMPEL™ are comprised of iron oxide crystals dispersed in a polymer matrix, with a functional polymer overcoating for the encapsulation of magnetite and the introduction of reactive groups. As they are polymer-based, they retain the low density that is advantageous for biomolecular assays. Our initial offerings include 3µm, 6µm, and 8µm beads with measured CVs of ≤ 5% and high levels of COOH-functionalization. Customers ordering our 0.25g offering will also receive a 1.5mL magnetic separator - absolutely free!

Fluorescent Magnetic Polymers

Both Classical (MC catalog codes) and Encapsulated (ME catalog codes) superparamagnetic microspheres are synthesized using a process that results in the distribution of iron oxide crystals throughout the bead matrix. The surfaces of Encapsulated magnetic microspheres are expected to be free of iron oxide, while the Classical version will have some exposed iron oxide. Fluorescent polymer microspheres are available in four standard volumes: 1mL, 5mL, 10mL, and 100mL.

BioMag® and BioMag®Plus

BioMag® Superparamagnetic Particles are high-performance superparamagnetic microparticles widely used in the magnetic separation of cells, organelles, proteins, immunoglobulins, nucleic acids, and many other types of molecules in biological and non-biological systems. Their irregular shape affords a much greater surface area than that of the same size spherical particle. This large surface area results in high binding capacities, allowing for efficient capture of target with minimum amounts of particles. BioMag® particles are greater than 90% iron oxide resulting in a fast magnetic separation, especially on high throughput automated platforms. The particles are covered with a coating that provides functional groups for the attachment of proteins or antibodies.

There are three varieties of BioMag® Superparamagnetic Microparticles: BioMag®, BioMag®Plus, and BioMag® Maxi. BioMag® particles have a mean diameter of ~1.5µm (with the exception of BioMag® Superparamagnetic Iron Oxide [~10µm]). BioMag®Plus particles are similar to conventional BioMag® particles with the important distinction of fewer fines. Additionally, BioMag®Plus particles are often offered in kits as the principle component. BioMag® Maxi particles have a mean diameter of ~3-12µm.

BioMag® and BioMag®Plus Unconjugated Particles

BioMag® and BioMag®Plus Unconjugated Particles are offered for the covalent attachment of proteins or ligands while allowing for the retention of biological activity.

BioMag® Magnetic Immobilization Kit

The BioMag® Magnetic Immobilization Kit is designed to introduce the user to the ease of magnetizing biological molecules. The kit includes BioMag® Amine particles, glutaraldehyde (reagent used to couple proteins to BioMag® particles), a BioMag® Flask Separator, and a Reaction Flask.

BioMag® mRNA Purification

Nuclease-free suspensions of BioMag® with covalently-bound oligo dT (20) are utilized for the efficient isolation of polyadenylated mRNA from total RNA, tissue, or cell lysate. Following isolation, mRNA is eluted through a simple incubation step in DEPC-treated water. BioMag® Oligo dT (20) particles may be purchased alone or as a component of our BioMag® mRNA Purification System.

BioMag® Streptavidin, Nuclease-free

Streptavidin-coated BioMag® particles are offered in a nuclease-free preparation for the binding or capture of biotinylated nucleic acids.

BioMag® and BioMag®Plus Binding Proteins

BioMag® particles with affinity binding proteins may be coated with the primary antibody or biotinylated molecule of interest through a straightforward incubation. They are also suitable for off-the-shelf use in applications, such as immunoprecipitation or PCR product clean-up.

BioMag® and BioMag®Plus Protein Isolation and Removal Kits

With their tremendous surface area, BioMag® particles are ideal for purifications. Our antibody isolation kits provide a means for the efficient isolation of antibody from serum or cell culture supernatant, and our protein removal kits permit the depletion of ubiquitous proteins, such as albumin and immunoglobulin from serum, for a significant improvement in resolution of less abundant proteins.

BioMag® and BioMag®Plus Secondary Antibodies

BioMag® secondary antibody particles are used extensively in the capture of immune complexes for immunoprecipitation and antibody-labeled cells for indirect cell separation. Secondary antibody coatings also afford a convenient method for binding specific primary antibodies to BioMag® particles.

BioMag®Plus Mouse anti-Fluorescein IgG

BioMag®Plus Mouse anti-Fluorescein IgG particles may be used to isolate fluorescein-tagged species, such as cells or nucleic acids.

BioMag® anti-Leukocyte for Human

BioMag® anti-Leukocyte particles are coated with anti-CD antibodies for the positive selection of specific cell subsets from purified leukocyte preps or whole blood. Positive selections permit rapid and efficient isolation of target cell populations.

BioMag® anti-Leukocyte for Mouse

BioMag® anti-Leukocyte particles are coated with anti-CD antibodies for the positive selection of specific cell subsets from purified leukocyte preps or whole blood. Positive selections permit rapid and efficient isolation of target cell populations.

BioMag® Human T cell Enrichment Systems

BioMag® Human T cell Enrichment Systems are available for the enrichment of CD3+, CD4+, or CD8+ T cell fractions from human peripheral blood mononuclear cells. Our enrichment systems involve the magnetic removal of subpopulations positive for other CD markers, leaving the untouched CD3+, CD4+, or CD8+ fraction available for analysis.

BioMag® Dextran-coated Charcoal

BioMag® Dextran-coated Charcoal is suitable for the adsorption of free tritium-labeled analyte in typical competitive radioimmunoassays. BioMag® particles are coated with NORIT® activated carbon and dextran.

BioMag® Superparamagnetic Iron Oxide

BioMag® Superparamagnetic Iron Oxide may be used for applications in which an iron oxide particle is required. Particles are ~10µm in size, and are non-functionalized.

BioMag® Maxi

BioMag® Maxi have the same structure and composition as standard BioMag® products, but with a larger mean diameter of ~3-12µm.

QuantumPlex™M

QuantumPlex™M (Magnetic) kits consist of five populations of ~6µm highly uniform superparamagnetic microspheres encoded with different intensities of Starfire Red™. Like QuantumPlex™, they are suitable for use on standard flow cytometers (488nm or 633nm excitation). Highly efficient separations may be performed using rare earth magnetic separators. Carboxyl (COOH) and streptavidin surfaces are available. QuantumPlex™M are available in three sizes: A (1mL/population - 5x100 data points), B (5mL/population - 5x500 data points), and C (10mL/population - 5x1000 data points).

QuantumPlex™M SP (Magnetic, Single Population) is useful for the development of simplex flow cytometric assays, or for the optimization of attachment chemistry and assay parameters before transitioning to a multiplexed format. Carboxyl (COOH) and streptavidin surfaces are available. QuantumPlex™M SP are available in two sizes: A (1mL - 100 data points) and B (3mL - 300 data points).