- Applications at a Glance
- Bargain Beads
- Dyed Microspheres
- Flow Cytometry Products
- QuickCal® Analysis Template
- Fluorescent Microspheres
- Magnetic Microspheres
- Magnetic Separators
- NIST Traceable Particle Size Standards
- Polymer Microspheres
- Proactive® Protein Coated Microspheres
- SNARe™ DNA Purification Systems
- Solutions and Buffers
- Uniform Silica Microspheres
- ViaCheck™ Viability Instrument Standards
Flow Cytometry Products
Full Spectrum™
Full Spectrum™ microspheres provide a convenient means for performing the initial daily QC check on 3-6 color instruments. These ~7-9µm microspheres are internally labeled with multiple fluorophores for use with common excitation and detection wavelengths within the visible spectrum. Full Spectrum™ are available in three sizes: A (20 tests), B (100 tests), and C (280 tests).
| Catalog Code | Description | Product Data Sheet | Material Safety Data Sheet |
|---|---|---|---|
| 885 | Full Spectrum™ | PDS 885.pdf | MSDS 885.pdf |
Fluorescence Reference Standards
Single-color Fluorescence Reference Standards are labeled with specific fluorochromes to exhibit the same spectral characteristics as labeled cells. Their size approximates that of human lymphocytes (~7-9µm in diameter) and are available in a wide vareity of fluorescent dyes. Both the fluorescein and propidium iodide are also available as 2µm beads. These standards may be used to QC a specific path of the optical system (laser/filter/PMT), to optimize filter and mirror sets for fluorophores, and to establish a test-specific Target Channel Value for instrument set-up. Fluorescence References Standards are available in three sizes: A (20 tests), B (100 tests), and C (280 tests).
Right Reference Standard™
Each standard consists of a population of microspheres surface labeled with a single fluorochrome at a given intensity level (low, medium, high). The Right Reference Standard™ microspheres approximate the size of human lymphocytes (~7-9µm). With their narrow fluorescence CVs, Right Reference Standard™ products are suitable for alignment purposes. These standards may also be used to QC a specific path of the optical system (laser/filter/PMT), and to establish a unified fluorescence range for a particular detector.
| Catalog Code | Description | Product Data Sheet | Material Safety Data Sheet |
|---|---|---|---|
| 510 | Right Reference Standard™ Fluorescein Low Level | PDS 510.pdf | MSDS 510.pdf |
| 511 | Right Reference Standard™ Fluorescein Medium Level | PDS 510.pdf | MSDS 511.pdf |
| 512 | Right Reference Standard™ Fluorescein High Level | PDS 510.pdf | MSDS 512.pdf |
| 513 | Right Reference Standard™ Phycoerythrin Low Level | PDS 510.pdf | MSDS 513.pdf |
| 514 | Right Reference Standard™ Phycoerythrin Medium Level | PDS 510.pdf | MSDS 514.pdf |
| 515 | Right Reference Standard™ Phycoerythrin High Level | PDS 510.pdf | MSDS 515.pdf |
| 516 | Right Reference Standard™ PE-Cy™5 Low Level | PDS 510.pdf | MSDS 516.pdf |
| 517 | Right Reference Standard™ PE-Cy™5 Medium Level | PDS 510.pdf | MSDS 517.pdf |
| 518 | Right Reference Standard™ PE-Cy™5 High Level | PDS 510.pdf | MSDS 518.pdf |
| 519 | Right Reference Standard™ APC Low Level | PDS 510.pdf | MSDS 519.pdf |
| 520 | Right Reference Standard™ APC Medium Level | PDS 510.pdf | MSDS 520.pdf |
| 521 | Right Reference Standard™ APC High Level | PDS 510.pdf | MSDS 521.pdf |
Ultra Rainbow Fluorescent Particles
Ultra Rainbow Fluorescent Particles (by Spherotech™) are internally labeled with multiple fluorophores, enabling excitation at any wavelength from 365-650nm. Ultra Rainbow Fluorescent Particles may be used for general QC purposes for 3-9 color instruments. Ultra Rainbow Fluorescent Particles are available in three sizes: A (20 tests), B (100 tests), and C (280 tests).
| Catalog Code | Description | Product Data Sheet | Material Safety Data Sheet |
|---|---|---|---|
| 610 | Ultra Rainbow Fluorescent Particles, ~3.8µm (3.61-3.99µm) | PDS 610.pdf | MSDS 610.pdf |
| 611 | Ultra Rainbow Fluorescent Particles, ~10.2µm (8.1-12.0µm) | PDS 611.pdf | MSDS 611.pdf |
QC Windows®
QC Windows® kits for instrument set-up include a blank and one or more bead population(s) surface-labeled with fluorochromes. The kits allow the establishment of upper and lower fluorescence thresholds, the Window of Analysis, for each detector, permitting meaningful data comparison between instruments and over time. These microspheres are ~7-9µm in diameter and approximate the size of human lymphocytes. QC Windows® kits are available in three sizes: A (20 tests), B (100 tests), and C (280 tests).
| Catalog Code | Description | Product Data Sheet | Material Safety Data Sheet |
|---|---|---|---|
| 845 | QC Windows® (FITC/PE) | PDS 845.pdf | MSDS 845.pdf |
| 846 | QC Windows® (FITC/PE/PE-TR) | PDS 845.pdf | MSDS 846.pdf |
| 847 | QC Windows® (FITC/PE/PE-Cy™5) | PDS 845.pdf | MSDS 847.pdf |
| 848 | QC Windows® (FITC/PE/PE-Cy™5, APC) | PDS 845.pdf | MSDS 848.pdf |
QC3™
QC3™ kits include one or more bead population(s) surface-labeled with fluorochromes for defining an upper fluorescence threshold for each detector, permitting meaningful comparion between instruments and over time. These microspheres are ~7-9µm in diameter and approximate the size of human lymphocytes. QC3™ kits are available in three sizes: A (20 tests), B (100 tests), and C (280 tests).
| Catalog Code | Description | Product Data Sheet | Material Safety Data Sheet |
|---|---|---|---|
| 841 | QC3™ (FITC/PE) | PDS 841.pdf | MSDS 841.pdf |
| 842 | QC3™ (FITC/PE/PE-TR) | PDS 841.pdf | MSDS 842.pdf |
| 843 | QC3™ (FITC/PE/PE-Cy™5) | PDS 841.pdf | MSDS 843.pdf |
| 844 | QC3™ (FITC/PE/PE-Cy™5, APC) | PDS 841.pdf | MSDS 844.pdf |
FITC/PE Compensation Standard
The FITC/PE Compensation Standard includes four microsphere populations that are ~7-9µm in diameter: an Autofluor™ population, and single populations surface-labeled with FITC, PE, and FITC/PE. The Autofluor™ population is dyed with a low level of fluorophore to approximate the autofluorescence of an unstained resting lymphocyte. This kit is useful for establishing quadrant boundaries and compensation settings for analyses using FITC and PE fluorochromes. The FITC/PE Compensation Standard is available in three sizes: A (20 tests), B (100 tests), and C (280 tests).
| Catalog Code | Description | Product Data Sheet | Material Safety Data Sheet |
|---|---|---|---|
| 820 | FITC/PE Compensation Standard (Autofluor™/FITC-PE/FITC/PE) | PDS 820.pdf | MSDS 820.pdf |
Simply Cellular® Compensation Standard
The Simply Cellular® Compensation Standard includes a mixed population of low- and high-binding antibody-coated beads that are ~7-9µm in diameter. Users label aliquots with antibody conjugates of interest to establish suitable compensation settings for a specific analysis.
| Catalog Code | Description | Product Data Sheet | Material Safety Data Sheet |
|---|---|---|---|
| 550 | Simply Cellular® Compensation Standard (anti-Mouse IgG) | PDS 850.pdf | MSDS 550.pdf |
| 551 | Simply Cellular® Compensation Standard (anti-Rat IgG) | PDS 851.pdf | MSDS 551.pdf |
Viability Dye Compensation Standard
Viability Dye Compensation Standards are suitable for labeling with LIVE/DEAD® stains or similarly-reactive dyes to generate compensation standards for flow cytometric analyses. Beads are not suitable for labeling with DNA stains such as propidium iodide, DAPI, or SYTOX®, and users should contact us for discussion if uncertain as to the compatability of a specific dye or stain.
| Catalog Code | Description | Product Data Sheet | Material Safety Data Sheet |
|---|---|---|---|
| 450 | Viability Dye Compensation Standard, 4µm | PDS 853.pdf | MSDS 450.pdf |
| 451 | Viability Dye Compensation Standard, 8µm | PDS 853.pdf | MSDS 451.pdf |
Quantum™ MESF
Our Quantum™ MESF kits include microsphere populations that are ~7-9µm in diameter: one blank population and a range of labeled bead populations with increasing amounts of fluorochrome. The assignment of fluorescence intensity in Molecules of Soluble Fluorochrome (MESF) units is performed through direct comparison of fluorescence measurements from solutions of the pure fluorochrome with those from microspheres surface-labeled with the same fluorochrome. In addition to quantitative fluorescence cytometry, Quantum™ MESF kits may be used to assess the detection threshold, resolution, and linearity of the instrument. A free QuickCal® analysis template is provided with each kit to aid in determining the expression level of cells, and for evaluating instrument linearity and detection threshold. Quantum™ MESF kits are available in three sizes: A (20 tests), B (100 tests), and C (280 tests).
Note to FITC MESF Users: We are now offering a single version of Quantum™ FITC MESF rather than the separate Low, Medium, and High Intensity kits. The new version consists of one blank and five labeled bead populations that span the full intensity range previously covered by the three separate kits. See PDS 855, Quantum™ FITC-5 MESF, or 856, Quantum™ FITC-5 MESF Premix, for more information on the kits, and PDS 819, QuickCal® v. 2.3 Data Analysis Program, for details regarding QuickCal® templates.
| Catalog Code | Description | Product Data Sheet | Material Safety Data Sheet |
|---|---|---|---|
| 555 | Quantum™ FITC-5 MESF | PDS 855.pdf | MSDS 555.pdf |
| 555p | Quantum™ FITC-5 MESF (Premix) | PDS 856.pdf | MSDS 555p.pdf |
| 827 | Quantum™ R-PE MESF | PDS 827.pdf | MSDS 827.pdf |
| 828 | Quantum™ PE-Cy™5 MESF | PDS 828.pdf | MSDS 828.pdf |
| 823 | Quantum™ APC MESF | PDS 823.pdf | MSDS 823.pdf |
Quantum™ Simply Cellular®
Quantum™ Simply Cellular® (QSC) kits are comprised of five microsphere populationsthat are ~7-9µm in diameter: one blank and four labeled with increasing amounts of antibody. Each coated population binds a specific number of monoclonal antibodies of the noted species, which is equal to its Antibody Binding Capacity (ABC) value. The investigator labels QSC beads using the same antibodies used to stain cells. In addition to quantitative fluorescence cytometry, QSC kits may be used for compensation. A free QuickCal® analysis template is provided with each kit to aid in determining the expression level of cells, and for evaluating instrument linearity and detection threshold. Quantum™ Simply Cellular® kits are available in three sizes: A (20 tests), B (100 tests), and C (280 tests).
| Catalog Code | Description | Product Data Sheet | Material Safety Data Sheet |
|---|---|---|---|
| 815 | Quantum™ Simply Cellular® anti-Mouse IgG | PDS 815.pdf | MSDS 815.pdf |
| 816 | Quantum™ Simply Cellular® anti-Human IgG | PDS 816.pdf | MSDS 816.pdf |
| 817 | Quantum™ Simply Cellular® anti-Rat IgG | PDS 817.pdf | MSDS 817.pdf |
Simply Cellular®
Our Simply Cellular® standard consists of a single population of antibody-coated microspheres (~7-9µm in diameter) of known Antibody Binding Capacity (ABC). The population is stained with the user's primary antibody and used in conjunction with a suitable Quantum™ MESF kit for determination of the antibody's effective Fluorophore:Protein (F/P) ratio. This standard may also be used to QC the fluorescence intensity of different antibody lots or clones. Simply Cellular® kits are available in three sizes: A (20 tests), B (100 tests), and C (280 tests).
| Catalog Code | Description | Product Data Sheet | Material Safety Data Sheet |
|---|---|---|---|
| 810 | Simply Cellular® anti-Mouse IgG | PDS 810.pdf | MSDS 810.pdf |
| 812 | Simply Cellular® anti-Human IgG | PDS 812.pdf | MSDS 812.pdf |
| 813 | Simply Cellular® anti-Rat IgG | PDS 813.pdf | MSDS 813.pdf |
Flow Cytometry Absolute Count Standard™
Our Flow Cytometry Absolute Count Standard™ is a precisely counted population of microspheres for estimating counts of unlabeled cells via flow cytometry. The microspheres are ~7-9µm in diameter. Beads are internally labeled with multiple fluorophores for excitation with common lasers (e.g. 488nm, 633nm) and discrimination from the cell population. By evaluting the ratio of microspheres to cells, the volumetric number of cells may be determined.
| Catalog Code | Description | Product Data Sheet | Material Safety Data Sheet |
|---|---|---|---|
| 580 | Flow Cytometry Absolute Count Standard™ | PDS 880.pdf | MSDS 580.pdf |
Size Calibration Standards Kit
The Size Calibration Standards Kit is comprised of five populations of undyed microspheres in the range of ~4-12µm. Forward scatter channel (FSC) values may be plotted against reported bead diameter to generate a curve that may be used for estimation of the sizes of cells run at the same instrument settings. The Size Calibration Standards Kits are available in two sizes: B (100 tests) and C (280 tests).
| Catalog Code | Description | Product Data Sheet |
|---|---|---|
| 829 | Size Calibration Standards Kit | PDS 829.pdf |
QuantumPlex™
QuantumPlex™ kits provide a platform for the design of multiplexed suspension arrays that may be run on standard flow cytometers (488nm or 633nm excitation). Microsphere populations in five-bead kits are encoded with different intensities of Starfire Red™, and microspheres in our ten-bead kits are distinguished by both fluorescence intensity and size. Starfire Red™ exhibits very little carryover into lower wavelengths, leaving other detectors available for determination of positive binding events via common reporters, such as FITC and PE. Carboxyl (COOH), streptavidin, and anti-Mouse IgG surfaces are available. QuantumPlex™ are available in three sizes: A (1mL/population - 5x100 data points), B (5mL/population - 5x500 data points), and C (10mL/population - 5x1000 data points).
| Catalog Code | Description | Product Data Sheet | Material Safety Data Sheet |
|---|---|---|---|
| 235 | QuantumPlex™ COOH 4.4µm, 5 populations | PDS 235.pdf | MSDS 235.pdf |
| 238 | QuantumPlex™ COOH 5.5µm, 5 populations | PDS 235.pdf | MSDS 238.pdf |
| 239 | QuantumPlex™ COOH 4.4µm and 5.5µm, 10 populations | PDS 235.pdf | MSDS 239.pdf |
| 215 | QuantumPlex™ Streptavidin 4.4µm, 5 populations | PDS 215.pdf | MSDS 215.pdf |
| 218 | QuantumPlex™ Streptavidin 5.5µm, 5 populations | PDS 215.pdf | MSDS 218.pdf |
| 219 | QuantumPlex™ Streptavidin 4.4µm and 5.5µm, 10 populations | PDS 215.pdf | MSDS 219.pdf |
| 205 | QuantumPlex™ anti-Mouse 4.4µm, 5 populations | PDS 209.pdf | MSDS 205.pdf |
| 208 | QuantumPlex™ anti-Mouse 5.5µm, 5 populations | PDS 209.pdf | MSDS 208.pdf |
| 209 | QuantumPlex™ anti-Mouse 4.4µm and 5.5µm, 10 populations | PDS 209.pdf | MSDS 209.pdf |
QuantumPlex™ SP
QuantumPlex™ SP (Single Population) is useful for the development of simplex flow cytometric assays, or for the optimization of attachment chemistry and assay parameters before transitioning to a multiplexed format. Like QuantumPlex™, they are dyed with Starfire Red™ and are suitable for use on standard flow cytometers and carboxyl (COOH), streptavidin, and anti-Mouse IgG surfaces are available. QuantumPlex™ SP are available in two sizes: A (1mL - 100 data points) and B (3mL - 300 data points).
| Catalog Code | Description | Product Data Sheet | Material Safety Data Sheet |
|---|---|---|---|
| 234 | QuantumPlex™ SP COOH 4.4µm | PDS 234.pdf | MSDS 234.pdf |
| 237 | QuantumPlex™ SP COOH 5.5µm | PDS 234.pdf | MSDS 237.pdf |
| 214 | QuantumPlex™ SP Streptavidin 4.4µm | PDS 214.pdf | MSDS 214.pdf |
| 217 | QuantumPlex™ SP Streptavidin 5.5µm | PDS 214.pdf | MSDS 217.pdf |
| 204 | QuantumPlex™ SP anti-Mouse 4.4µm | PDS 207.pdf | MSDS 204.pdf |
| 207 | QuantumPlex™ SP anti-Mouse 5.5µm | PDS 207.pdf | MSDS 207.pdf |
QuantumPlex™M
QuantumPlex™M (Magnetic) kits consist of five populations of ~6µm highly uniform superparamagnetic microspheres encoded with different intensities of Starfire Red™. Like QuantumPlex™, they are suitable for use on standard flow cytometers. Highly efficient separations may be performed using rare earth magnetic separators. Carboxyl (COOH) and streptavidin surfaces are available. QuantumPlex™M are available in three sizes: A (1mL/population - 5x100 data points), B (5mL/population - 5x500 data points), and C (10mL/population - 5x1000 data points).
| Catalog Code | Description | Product Data Sheet | Material Safety Data Sheet |
|---|---|---|---|
| 250 | QuantumPlex™M COOH, 5 populations | PDS 250.pdf | MSDS 250.pdf |
| 252 | QuantumPlex™M Streptavidin, 5 populations | PDS 252.pdf | MSDS 252.pdf |
QuantumPlex™M SP
QuantumPlex™M SP (Magnetic, Single Population) is useful for the development of simplex flow cytometric assays, or for the optimization of attachment chemistry and assay parameters before transitioning to a multiplexed format. Like QuantumPlex™M, they are ~6µm highly uniform superparamagnetic microspheres dyed with Starfire Red™ and are suitable for use on standard flow cytometers. Carboxyl (COOH) and streptavidin surfaces are available. QuantumPlex™M SP are available in two sizes: A (1mL - 100 data points) and B (3mL - 300 data points).
| Catalog Code | Description | Product Data Sheet | Material Safety Data Sheet |
|---|---|---|---|
| 251 | QuantumPlex™M SP COOH | PDS 251.pdf | MSDS 251.pdf |
| 253 | QuantumPlex™M SP Streptavidin | PDS 253.pdf | MSDS 253.pdf |
QuickCal® Analysis Template, v. 2.3
QuickCal® constructs a calibration curve associating fluorescence channel values to standardized fluorescence intensity units. The channel value corresponding to the kit's blank bead is converted to the appropriate standardized fluorescence intensity unit (MESF or ABC); this will be the instrument's detection threshold for the specific reporter and detector. For instructions regarding QuickCal, please see Product Data Sheet 819, QuickCal® v. 2.3 Data Analysis Program.